MALDI-TOF MS: Much More than only Protein Analysis!
نویسندگان
چکیده
منابع مشابه
MALDI-TOF-MS analysis of protein and DNA.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has become a powerful and widespread analytical tool in all fields of life science. The wide mass range (1-300 kDa), high accuracy, and sensitivity make it a superior method for analysis of all kinds of biomolecules including proteins, nucleic acids, and carbohydrates. In combination with 2D-gelelectroph...
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Matrix-assisted laser desorption (MALDI) time-offlight (TOF) mass spectrometry (MS) has been successfully used for oligonucleotide sequence confirmation by mass analysis of fragments generated by concentration-dependent exonuclease digestion. This approach is particularly well-suited to the analysis of synthetic DNA due to the relatively short length of the DNA strands. Additionally, an importa...
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Hepatocellular carcinoma (HCC) is one of the most common types of cancer worldwide. The initial hepatocellular alterations that precede the appearence of HCC include chronic viral hepatitis/cirrhosis, foci of phenotypically altered hepatocytes and, subsequently, dysplastic hepatocytes that form foci and nodules. These changes cause a discrepancy in the microenvironment of liver cells, which may...
متن کاملOptimization of magnetic beads for maldi-TOF MS analysis.
MALDI-TOF MS is being widely applied to analyze serum samples for the diagnosis of human diseases and for the identification of potential biomarkers. However, different types of magnetic beads afford different purification capability for low abundance proteins and peptides in body fluids. The aim of our study was to establish a proteome fractionation technique for choosing magnetic beads for pr...
متن کاملMALDI-TOF MS in Prenatal Genomics.
Prenatal diagnosis aims either to provide the reassurance to the couples at risk of having an affected child by timely appropriate therapy or to give the parents a chance to decide the fate of the unborn babies with health problems. Invasive prenatal diagnosis (IPD) is accurate, however, carrying a risk of miscarriage. Non-invasive prenatal diagnosis (NIPD) has been developed based on the exist...
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ژورنال
عنوان ژورنال: Journal of Glycomics & Lipidomics
سال: 2013
ISSN: 2153-0637
DOI: 10.4172/2153-0637.1000e113